 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Characterization of Multiple-Antimicrobial Resistant Salmonella Isolated from Pig Farms in Thailand) ผู้เขียน: พรรณนที แสนพงษ์,  ดร.กัญจนา ธีระกุล, รองศาสตราจารย์ , Worrawit Wajjwalku, ดร.ปฐมาพร เอมะวิศิษฎ์, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractA cross-sectional study was conducted to characterize Salmonella enterica serogroup B and C in five intensive pig farms in central Thailand. Of 230 Salmonella isolates, 211 isolates (91.74%) were resistant to three or more antimicrobials and were defined as MDR isolates. Every isolate (100%) was resistant to sulfamethoxazole. The isolates with high resistance rates (95, 91 and 78%) were resistant to tetracycline, ampicillin and streptomycin, respectively. All of the isolates were sensitive to ceftriaxone, ceftriofur and ciprofloxacin. Only four of the 211 MDR isolates harbored class 1 integrons that carried aadA gene cassettes, which conferred resistance to streptomycin. Each isolate of serovar S. Stanley and S. Anatum harbored the aadA1 and the aadA2 gene cassette, respectively, and two isolates of serovar S. Panama contained the aadA4 gene cassette. These four isolates could transfer resistance genes and class 1 integron carrying the aadA gene to E. coli by conjugative plasmids. The common antimicrobial resistances that were found in transconjugants were ampicillin, chloramphenicol, kanamycin, sulfamethoxazole and tetracycline. The high occurrence of Salmonella on one farm presented three common serovars, namely S. Corvallis, S. Rissen, and S. 1,4,5,12:i-. The antimicrobial resistance pattern was the same in each serovar. Their PCR-RFLP of flagellin genes and plasmid profiles showed that these three serovars were possibly endemic strains on this farm and had spread by cross contamination. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : PCR-based Restriction Fragment Length Polymorphism for Subtyping of Salmonella from Chicken Isolates) ผู้เขียน:Han Yu Jong, Pak Thae Su, พรรณนที แสนพงษ์, วรวิทย์ วัชชวัลคุ, Thavajchai Sukpuaram, ดร.ปฐมาพร เอมะวิศิษฎ์, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractGenotypic diversity in two flagellin genes, fliC and fljB, encoding phase-1 and phase-2 flagellin of Salmonella enterica, offers a potential biomarker for Salmonella subtyping. Forty-seven Salmonella isolates of 20 different serovars derived from chicken samples in Thailand were studied using the fliC/ fljB PCR-based RFLP assay. With two restriction endonucleases, MboI and HhaI, the fliC showed 11 and 9 patterns, while the fljB showed 6 and 7 patterns respectively. Though the PCR-based RFLP test cannot replace serotyping, the assay is based on the flagellin genes encoding proteins on the bacterial surface that are related to serotyping scheme. Overall, the assay was reproducible and successfully applied to simply screen Salmonella serovars as an alternative subtyping test for rapid traceability of Salmonella contamination in chicken production. |